This chapter summarizes the ideas and procedures used in the analysis of brain imaging data. Friston, in Statistical Parametric Mapping, 2007 INTRODUCTION Valeria Samarelli and Marco Arese, Associate Professor of Biochemistry, Department of Oncological Sciences - University of Turin - School of Medecine - Institute for Cancer Research and Treatment (IRCC). Université de Genève, Université de Lausanne Sciences II. Pharmacognosie, Phytochimie et produits naturels bioactifs. Web: ĭr Sarah Berndt and Prof Muriel Cuendet. of Radiology and Oncology, The Johns Hopkins University School of Medicine, Baltimore. Development and Regeneration KU Leuven, Kortrijk. IPRASENSE, Cap Alpha, Clapiers, France.ĭacha Gholobova, Lieselot Decroix and Prof. CEA-Leti - MINATEC Campus, Grenoble, France. INSERM U1197, Hôpital Paul Brousse, Villejuif. Maria Bondesson Intelligent Systems Engineering, Indiana University, Bloomington, IN, USA.ĭr. Rachna Mirchandani, Savini Thrikawala, Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling, University of Houston, Houston, TX, USA, and Dr. Richard Cook and Pedro Bastos, Dental Institute, Guy’s Hospital, King’s College London, UK. The same analysis can be obtained using a fluorescent staining by calcein.Ĭollaborations - image and thematic providers:Įlisabeth VAGANAY et Forence RUGGIERO, Matrix Biology and Pathology, Institut de Génomique Fonctionnelle de Lyon, ENS de lyon, Université de Lyon, CNRS UMR 5242.ĭr. The Angiogenesis Analyzer is a simple tool to quantify the ETFA experiment images by extracting characteristic information of the network.Ībove: phase contrast image of HUVEC network, analyzed by the Angiogenesis Analyzer for ImageJ. Although widely used, the interpretation of this assay still remains a problem, especially to obtain a quantitative evaluation of the vessels-like net organization.
At later stage this differentiation can lead to a meshed network from different mesh sizes. In suitable culture conditions these cells form structures that can branch and mimic a pseudo capillary in vitro formation. Typically, it can detect and analyze the pseudo vascular organization of endothelial cells cultured in gel medium.Īmong the tools used to evaluate the anti-angiogenesis properties of drugs, the most used is the in vitro differentiation of primary endothelial cells culture in gel (Endothelial Tube Formation Assay (ETFA)). The " Angiogenesis Analyzer" allows analysis of cellular networks.
0 kommentar(er)
